Prof. Fred Wouters
Our research focusses on the working and regulating of cellular molecular machines in health and disease. Examples include tyrosine kinase receptor signalling, programmed non-apoptotic cell death signalling and the mechanisms involved in inhibitory synapse formation. Mechanistic insight is obtained by the use of advanced quantitative fluorescence microscopy techniques. These primarily include Fluorescence Lifetime Imaging Microscopy (FLIM), and Förster Resonance Energy Transfer (FRET) microscopy. Furthermore, we design targeted optical assays for specific biological functions and activities with a focus on multiplexed detection in order to discover and/or describe connections and causalities.
In close collaboration with the UMG technology platform for Clinical Optical Microscopy (CLINOMIC) of Dr. Bunt, we recently started the development of volumetric deep tissue imaging techniques for use with human pathological tissue. Recent advances include a fast and effective method for optical clearing of large volumes of formaldehyde fixed pathological tissue and the development of a light sheet fluorescence microscope for cm-sized samples with 3-dimensional cellular resolution. With the implementation of advanced imaging techniques in pathology, we aim to introduce new and improved optical tools for clinical diagnosis.